3D culture systems present an important advancement in cell culture techniques. Constructed from cell lines or primary cells, the 3D models simulate in vivo situations and present invaluable systems for fundamental and applicative cell biology research in vitro. Attendees at this workshop will see presentations on multicellular 3D models and their characterisation from an academic and industrial point of view, as well as receive hands-on training in the assembly of lung, intestine and skin models.