Taking place from October 1-4, 2019 in Engelberg, Switzerland, the Interdisciplinary Symposium on 3D Microscopy focuses on 3D microscopic imaging and spectroscopy in science. The objective of this symposium is to create a forum for researchers with different expertise and scientific interests to present their knowhow and the techniques they use to answer their scientific questions. Methods using three-dimensional imaging and spectroscopy to retrieve data in volume will be discussed, whether “light”, x-rays or electrons are used.