Researchers from the Adolphe Merkle Institute’s BioNanomaterials group have created a 3D lung cell model system to investigate how carbon-based combustion byproducts behave as they interact with human epithelial tissue.
Epidemiological studies have established a strong correlation between inhaling ultrafine particles from incomplete combustion and respiratory and cardiovascular diseases. Still, relatively little is known about the mechanisms behind how air particulates affect human health. Novel research with carbon nanodots now seeks to provide the first model of how ultrafine carbon-based particles interact with the lung tissues.
In a study published in the journal Biointerphases, the AMI investigators discovered that the surface properties of the carbon nanodots and aggregation patterns affected their distribution in a lab-grown copy of the lung's barrier layer, the epithelium. They used carbon nanodots as representatives for air pollution particles.
"Localization and quantification of inhaled carbon nanoparticles at the cellular level has been very difficult," said Prof. Barbara Rothen-Rutishauser, one of the authors of the paper, which is part of a special focus issue of Biointerphases on Women in Biointerface Science. "We now have a model fluorescent particle that can try to answer questions about the fate of ultrafine particles in the lung."
At less than 100 nanometers in diameter, ultrafine particles have the small size and large relative surface area to wreak havoc on cells and potentially enter the bloodstream. Other groups' research has shown that ultrafine particles induce adverse effects on the lungs and cardiovascular system by increasing oxidative stress in the body.
Because of particle size, it is difficult for lab techniques to distinguish between carbon in pollutants from carbon in tissues. Therefore, little is known about surface charge and states of agglomeration, two key physical and chemical features that affect how carbon particles interact with living tissues.
To begin modeling ultrafine particles, Dr. Estelle Durantie, another of the study’s authors, turned to fluorescent carbon nanodots doped with nitrogen and a combination of nitrogen and sulfur with different sizes and charges. The team then applied these nanodots to the top layer of a lab-grown epithelial tissue, where gas exchange typically happens in the lung.
Since regular fluorescent microscopes lack the resolution to visualize such small particles, the group used spectroscopy and UV light to detect and quantify nanodots as they migrated from the luminal compartment past their lung model's immune cells. As the researchers expected, charged particles tended to stick together before penetrating the gas-exchange barrier. While most of the neutrally charged nanodots passed through the tissue after only an hour, only 20 percent of the agglomerated charged particles infiltrated the epithelium.
Rothen-Rutishauser said she hopes to further improve nanodots so that they better mimic ultrafine particles. "What we're seeing is that translocation depends on aggregation state," she added. "We hope to continue trying out different sizes of nanodots, including other types of particles that get us closer to the real environment."
Original press release from the American Institute of Physics here.