Micro- and nanoplastics (MNPs) are emerging contaminants infiltrating ecosystems and the human body, with their impact still under investigation. This project develops advanced analytical tools—combining spectroscopy, imaging, and machine learning—to detect and characterize MNPs at ng/mL levels in complex matrices such as seawater and blood. By overcoming current limitations in size resolution, chemical specificity, and matrix interference, we aim to deliver rapid, high-precision detection strategies to support regulation and safeguard environmental and human health.